Transient Transfection

Your protein from mammalian in large scale within weeks

The recent rush in biopharmaceutical research and the advances in diagnostic assay development raised the need for significant amounts of correctly folded, fully processed and functional active recombinant tool proteins or preclinical material. While the use of stable transfected cell lines has a long-standing and successful history for the generation of biopharmaceutical proteins, these expression systems were generally regarded as being cumbersome, tedious and expensive. These drawbacks have been overcome by the recent development of large-scale transient transfection.

Our Welcome Offer

We’re ready to do a Feasibility Study with your recombinant Protein in small scale to provide you with an individualized quote.

This Feasibility Study includes following services:

  • Synthesis of cDNA
  • Cloning of cDNA into special proprietary vectors for transient transfection 
  • Plasmid preparation
  • Transient transfection: Cultivation of HEK293 cells in serum free suspension culture
  • Affinity purification (IMAC)
  • QC: protein conc. (UV 280 nm), capillary electrophoresis
  • Documentation: Certificate of Analysis
Price: € 2,000
additional: costs for synthesis of cDNA (extern)
You get the hole yield of the production.
 
Request a Quote via mail transient@invivo.de
or give us a call: +49 (0) 3302 883 769

 

Production Systems

Our optimized transfection protocol can be applied to adherent as well as serum-free suspension cultures. While adherent cells are transfected in „Cell Factories”, suspension cells like HEK293 are grown in square bottles, spinners or fermenters. Operation scales vary from ml-tubes to final production volumes of 30 liters in our bioreactor systems.

With the experience of the production and purification of more than 1800 different monoclonal antibodies and the successful completion of nearly two hundred recombinant protein projects we are confident to solve your problem.

Obtain your mammalian cell derived protein in the same time as you would expect it for a bacterial expressed product.    

InVivo, an independent contract manufacturer with over ten years experience:

InVivo is a leading contract manufacturing organization (CMO) dedicated to the development and production of monoclonal antibodies.

Based in Hennigsdorf, Germany, just outside Berlin, InVivo is an ISO 9001 certified company with over ten years experience in mammalian cell culture and protein production. As a trusted provider of cost-effective outsourcing solutions, InVivo has worked with a large number of satisfied clients. Companies of all sizes, including small university spin-offs, major research institutes, and biopharmaceutical companies, have taken advantage of our expertise as an independent contract manufacturer.

Rely on us for your diagnostic or biomedical outsourcing needs.

 Quality: With InVivo, you know what you get

As a leading supplier to the diagnostic industry, InVivo is committed to the highest standards of quality. We are dedicated to achieving the ultimate level of customer satisfaction and to the continual improvement of our products, services, and quality management system. To provide customers with products and services meeting their expectations and needs, InVivo has implemented and maintains a mandatory quality management system.

InVivo is able to provide endotoxin-free proteins on demand for use in preclinical trials as well as cGMP compliant documentation by the means of established procedures.

Any questions? Please contact us via mail transient@invivo.de
or give us a call: +49 (0) 3302 883 769