CD8β recAb (YTS156.7.7) InVivoPure+
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CD8β recAb (YTS156.7.7) InVivoPure+

ID: RP_SZ_1011P+ Categories: ,

CD8αβ acts as a co-receptor with peptidebound class I major histocompatibility complex (pMHC) on antigen-presenting cells (APC) for TCR-mediated activation of cytotoxic T cells.

The recombinant antibody is produced exclusively under serum-free (chemically defined, animal component free) conditions in human embryonic kidney (HEK) cells and purified with Protein-A or Protein-G affinity chromatography.

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Delivery time: 2-5 days

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Endotoxin level ≤ 1 EU/mg

The CD8β recAb (YTS156.7.7) reacts with the ~25 kDa beta subunit of mouse CD8αβ, also known as Ly-3, Lyt-3, CD8β or CD8 beta subunit. CD8αβ acts as a co-receptor with peptidebound class I major histocompatibility complex (pMHC) on antigen-presenting cells (APC) for TCR-mediated activation of cytotoxic T cells.
CD8β recAb (YTS156.7.7) blocks this interaction, playing an essential role in immune responses against internal and external offenses. The CD8β mAb (YTS156.7.7) is widely used as a phenotypic marker for mouse CD8αβ on cytotoxic T cells.
The original CD8β antibody (clone YTS156.7.7) is of rat IgG2b isotype. The present CD8β recAB (YTS156.7.7) antibody is designed as a chimeric antibody with wildtype mouse IgG2a kappa isotype, resulting in an anti-mouse CD8β recAb (YTS156.7.7) mouse IgG2a recombinant antibody.
The recombinant antibody is produced exclusively under serum-free (chemically defined, animal component free) conditions in human embryonic kidney (HEK) cells and purified with Protein-A or Protein-G affinity chromatography.

 

Product-ID: RP_SZ_1011P+
Clone: YTS156.7.7
Immunogen: Mouse CD8 (lyt-3)
Host: Mammalian; HEK
Isotype: Mouse IgG2a k
Formulation: Clear Liquid, PBS, pH 7.4, 0.2 μm sterile filtered
Concentration: ≥ 1.00 mg/mL
Purity: ≥ 95 % (CGE, reducing conditions)
≤ 5 % aggregates (analytical SEC)
Endotoxin: ≤ 1 EU/mg (LAL test)
Storage: 2 - 8 °C
Recommended Isotype Control: Mouse IgG2a ĸ Isotype Control (AK3399P+)
Reported Application: FACS; IHC; IF; Fn

 

The product is for research use only and not for use in diagnostic or therapeutic procedures.

 

Additional information

Literature

[1] Goodall KJ, Nguyen A, Andrews DM, Sullivan LC. Ribosylation of the CD8αβ heterodimer permits binding of
the nonclassical major histocompatibility molecule, H2-Q10. J Biol Chem. 2021 Oct;297(4):101141. doi:
10.1016/j.jbc.2021.101141. Epub 2021 Aug 31. PMID: 34478713; PMCID: PMC8517849.
[2] Shore DA, Issafras H, Landais E, Teyton L, Wilson IA. The crystal structure of CD8 in complex with YTS156.7.7
Fab and interaction with other CD8 antibodies define the binding mode of CD8 alphabeta to MHC class I. J
Mol Biol. 2008 Dec 31;384(5):1190-202. doi: 10.1016/j.jmb.2008.09.069. Epub 2008 Oct 7. PMID: 18929574;
PMCID: PMC2631232.
[3] Nakayama K, Nakayama K, Negishi I, Kuida K, Louie MC, Kanagawa O, Nakauchi H, Loh DY. Requirement for
CD8 beta chain in positive selection of CD8-lineage T cells. Science. 1994 Feb 25;263(5150):1131-3. doi:
10.1126/science.8108731. PMID: 8108731.
[4] van der Merwe PA, Davis SJ. Molecular interactions mediating T cell antigen recognition. Annu Rev Immunol.
2003;21:659-84. doi: 10.1146/annurev.immunol.21.120601.141036. Epub 2001 Dec 19. PMID: 12615890.
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Product Information Sheet – RP_SZ_1011P+
Notice

The product is for research use only and not for use in diagnostic or therapeutic procedures.

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