Endotoxin level ≤ 1 EU/mg
The HLA-A,B,C mAb (W6/32) monoclonal antibody reacts with the human major
histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta
2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated
immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic
epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43
kDa and 11-12 kDa chains. Crossreactivity is also seen in baboon, rhesus and cynomolgus
monkey.
MHC class I plays a crucial role in the adaptive immune response by presenting endogenous
antigens to cytotoxic CD8 T cells. The T cell receptor (TCR)/CD3 complex of CD8 T cells
interacts with peptide MHC class I, which induces CD8 T cell activation and subsequent cell
killing. CD8 molecules also bind to MHC class I, which enhances TCR signaling. In contrast to
CD8 T cells, MHC class I is an inhibitory ligand for natural killer cells (NK cells) and promotes selftolerance.
This antibody is produced exclusively under serum-free conditions from hybridoma and
purified with Protein-A or Protein-G affinity chromatography.
Applications Reported: This W6/32 antibody has been reported for use in flow cytometric
analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections.
| Product-ID: |
AK3603P+ |
| Clone: |
W6/32 |
| Immunogen: |
Animals were immunized with cells from human tonsils. Spleen cells were fused with P3X63Ag8 myeloma cells |
| Host: |
Mouse |
| Clonality: |
Monoclonal |
| Isotype: |
IgG2a κ |
| Formulation: |
Clear Liquid, PBS, pH 7.4, 0.2 μm sterile filtered |
| Concentration: |
≥ 1.00 mg/mL |
| Purity: |
≥ 95 % (CGE, reducing conditions) |
|
≤ 5 % aggregates (analytical SEC) |
| Endotoxin: |
≤ 1 EU/mg (LAL test) |
| Storage: |
2 - 8 °C |
| Recommended Isotype Control: |
Mouse IgG2a ĸ Isotype Control (AK3399P+) |
The product is for research use only and not for use in diagnostic or therapeutic procedures.
The HLA-A,B,C monoclonal antibody (clone W6/32) was purified from supernatant of HB- 95™ W6/32 from the ATCC. The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection.
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