PD-1 recAb (RMP1-14), mIgG2a κ, LALA-PG, InVivoPure+
PD-1 is expressed on the surface of activated T cells, B cells and myeloid cells. The present PD-1 recAb (RMP1-14) LALA-PG variant is based on the rat anti mouse PD-1 monoclonal antibody, clone RMP1-14 [PD-1 mAb (RMP1-14), InVivo Pure/+ (AK3615P/AK3615P+)].
The recombinant antibody is produced exclusively under serum-free (chemically defined, animal component free) conditions in human embryonic kidney (HEK) cells and purified with Protein-A or Protein-G affinity chromatography.
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Endotoxin level ≤ 1 EU/mg
PD-1 is expressed on the surface of activated T cells, B cells and myeloid cells. Blocking PD-1/PDL1 interaction promotes immune response against tumor cells. RMP1-14 is a monoclonal antibody that targets the murine PD-1 protein and has been used extensively to probe the effects of PD-1 inhibition in preclinical murine models.
The present PD-1 recAb (RMP1-14) LALA-PG variant is based on the rat anti mouse PD-1 monoclonal antibody, clone RMP1-14 [PD-1 mAb (RMP1-14), InVivo Pure/+ (AK3615P/AK3615P+)]. This initial antibody is of rat IgG2a isotype. The present PD-1 recAb (RMP1-14) LALA-PG antibody is designed as a chimeric antibody with modified mouse IgG2a kappa isotype. The antibody backbone (Fc) is modified to minimize unwanted immune responses and off-target effects in vivo. The antibody backbone is engineered with three point mutations in the Fc-region of the heavy chain: L234A / L235A / P329G = LALA-PG, according to Eu numbering convention). These mutations significantly reduce the binding of the antibody's Fc region to Fc gamma receptors (FcγRs) and reduces Fc-mediated toxicity in therapeutic / in vivo application.
The recombinant antibody is produced exclusively under serum-free (chemically defined, animal component free) conditions in human embryonic kidney (HEK) cells and purified with Protein-A or Protein-G affinity chromatography.
| Product-ID: | RP_SZ_1013P+ |
|---|---|
| Clone: | RMP1-14 mIgG2a k LALA-PG |
| Immunogen: | Mouse PD-1 transfected BHK cells |
| Expression: | Mammalian; HEK |
| Isotype: | Mouse IgG2a κ |
| Formulation: | Clear Liquid, PBS, pH 7.4, 0.2 μm sterile filtered |
| Concentration: | ≥ 1.00 mg/mL |
| Purity: | ≥ 95 % (CGE, reducing conditions) |
| ≤ 5 % aggregates (analytical SEC) | |
| Endotoxin: | ≤ 1 EU/mg (LAL test) |
| Storage: | 2 - 8 °C |
| Recommended Isotype Control: | Mouse IgG2a ĸ Isotype Control (AK3399P+) |
| Reported Application: | in vivo; WB; Flow Cytometry; Fn |
The product is for research use or for further manufacturing only and not for use in diagnostic or therapeutic procedures.
Additional information
| Literature | [1] Timilsina HP, Arya SP, Tan X. Biotechnological Advances Utilizing Aptamers and Peptides Refining PD-L1 |
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| Document | |
| Notice | The product is for research use or for further manufacturing only and not for use in diagnostic or therapeutic procedures. |
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